ГЕКСАГИДРАТ СПЕРМИДИНФОСФАТА

Description

Spermidine phosphate hexahydrate is a crystalline hydrate of the phosphate salt of the natural polyamine spermidine. The formula C14H59N6O18P3 given on the card corresponds to a composition of two spermidine molecules, three phosphate residues and six molecules of water of crystallization, which is consistent with the name “dispermidine triphosphate hexahydrate”. In the original catalog, the product is classified under the “Phosphines” section, but chemically it is a salt of phosphoric acid, not phosphine: there are no phosphorus-carbon bonds in the molecule.

Basic Attributes

• CAS No.: 23273-82-7
• Chemical formula: C14H59N6O18P3
• Molecular weight: 692.57
• Exact weight: 692.31000
• English name: SPERMIDINE PHOSPHATE HEXAHYDRATE

Synonyms

Dispermidine triphosphate hexahydrate; spermidine phosphate hexahydrate; spermidine phosphate salt hexahydrate; N-(3-aminopropyl)-1,4-diaminobutanophosphate hexahydrate; N-(3-aminopropyl)tetramethylenediamine phosphate hexahydrate; N-(3-aminopropyl)-1,4-butanediamine phosphate hexahydrate; N-[3-aminopropyl]-1,4-butanediamine phosphate salt hexahydrate.

Physico-chemical characteristics

• PSA: 446.23000
• Storage conditions: −20 °C

The polar surface area of ​​over 440 Ų is a direct consequence of the presence of three phosphate groups and six amino groups: the salt is highly hydrophilic and highly soluble in water. Storage at −20 °C, more stringent than 2–8 °C for the hydrochloride forms of polyamines, is due to the presence of water of crystallization: at room temperature, water loss and caking are possible.

Practical significance of phosphate form

Spermidine phosphate is used where the presence of chloride ion is undesirable: in crystallization tests, in buffer systems with a phosphate background, when preparing complexes of polyamines with nucleic acids. Spermidine itself, as a polycation, binds to the phosphate backbone of DNA and RNA, participates in cell proliferation, nucleic acid synthesis, apoptosis and protection from oxidative damage, and also serves as a donor of the aminobutyl group in the formation of hypusine in the translation factor eIF5A.

Scope of application

• reagent for crystallization of nucleic acids and nucleoprotein complexes
• component of buffer systems in biochemical experiments
• starting material in works on the chemistry and biology of polyamines
• standard sample for spermidine determination

Safety Information

• Transport codes: NONH for all modes of transport
• WGK Germany: 3
• Hazard class code: 36/37/38
• Safety instructions: 26-36
• Tags: Xi
• Hazard statements: H315 - skin irritation; H319 - serious eye irritation; H335 - may cause respiratory irritation
• Precautions: P261, P305+P351+P338

Phrase H335 and measure P261 define the hanging mode: inhalation of dust is avoided, work is carried out in a fume hood or with a respirator.

Storage and handling

Freezer compartment −20 °C, container tightly closed. Before opening the bottle, bring it to room temperature, otherwise moisture will condense on the cold crystalline hydrate. Class WGK Germany 3 requires that solutions not be discharged into the sewer system. Gloves and safety glasses are required.